電気インピーダンストモグラフィ（EIT）を用いた細胞凍結過程の可視化に関する基礎的検討

抄録

We report the results of a preliminary trial to visualize a cell during freezing process using microscopic electrical impedance tomography (micro-EIT). A printed circuit board was developed for micro-EIT with two measurement chambers, 5mm in diameter and 1.6mm in depth. These chambers were filled with ethanol (2mS/cm) which was then kept at -30^°C by placing it on a copper block filled with liquid nitrogen and by using a heater with a programmable controller. A grain of Citrus Sudachi and an egg of Cypselurus Agoo Agoo were placed into the measurement chamber and were recorded data during freezing by using a CCD microscope and micro-EIT. When the sample started freezing, the brightness of the sample became higher and the relative impedance in micro-EIT image also increased. Similar results were obtained in all the four trials respectively. Therefore, these results indicate that EIT is useful to observe freezing process of a cell.