MULTIVARIATE OPTIMIZATION OF ULTRASOUNDASSISTED EXTRACTION FOR PHYTOCHEMICALS DETERMINATION IN THAI SWEET PLANT “CHA-EM THAI” (ALBIZIA MYRIOPHYLLA)

抄録

The stems of Albizia myriophylla or Cha-em Thai have sweet compounds, which can be extracted by the ultrasound-assisted extraction (UAE) method. This study was to optimize the UAE conditions for maximum Chaem Thai extract relating to the saponin, flavonoid and sugar contents, using box-behnken design (BBD) with response surface methodology (RSM). Three factors and three levels including extraction temperature (X1), sonication time (X2) and extraction ratio (X3) were the independent variables used to optimize the extraction process. The optimized condition for UAE was 64ºC for temperature, 44 min for sonication time and 1:11 g/mL for extraction ratio. At this condition, the experimental values of saponin (1.5920 ± 0.0235 mg/gDW) was closely the predicted values (1.6590 mg/gDW). In addition, UAE extracts showed excellent DPPH radical scavenging activity. The BBD with RSM optimization study showed that the ultrasound-assisted extraction gave higher yield of sweetness compounds from Cha-em Thai than the conventional method.