Abstract
Release process of enzymes from baker's yeast accompanied by inactivation was experimentally investigated by using an agitating bead mill. The enzymes examined were Alkaline phosphatase and alcohol dehydrogenase. As a result, the disruption rate of yeast was directly proportional to the product of the concentration of undisrupted yeast and agitating speed of impeller, and the disruption process of yeast was clarified by this analysis. In order to investigate the inactivation process of enzymes, the activity of enzymes within the yeast was measured using a lytic method, the results of which are not influenced by the mechanical force. In the case where there is no inactivation, the release rate of enzymes was directly proportional to the product of the activity of unreleased enzymes and agitating speed of impeller. In the case of inactivation, the release rate of enzymes was directly proportional to the product of the activity of uninactivated enzymes and agitating speed of impeller. The experimentally analyzed models based on the kinetics were found to describe well the release process of enzymes accompanied by the inactivation from baker's yeast, disrupted by the agitating bead mill.
