Abstract
A HPLC method has been developed that can easily and sensitively separate and analyze amyloid-β aggregates. In this method, PTFE tubing (1000 mm × 0.50 mm i.d.) is used as the separation field. Furthermore, thioflavin T, a fluorescent detection reagent for amyloid fibrils, was added to the mobile phase. In this method, separation and labeling are carried out within a single channel from the sample injector to the detector, making it possible to achieve selective and highly sensitive online detection. Furthermore, the simulation and the experimental results suggest that the separation mechanism of this method may involve the Tubular Pinch Effect, a phenomenon in fluid dynamics. These results suggest that this method may be applicable to amyloid fibrils formed by proteins other than amyloid-β.
