2017 Volume 45 Issue 3 Pages 199-206
Oxidative stress is implicated in the pathogenesis of schizophrenia. Although atypical antipsychotic drugs have been reported to have antioxidant effects, the pathophysiologic mechanism of schizophrenia with various clinical symptoms and the effect of antipsychotics on neurons are entirely unknown. The aim of this study was to investigate the mechanism of aripiprazole, an atypical antipsychotic drug, on neurons. A shotgun proteomics analysis of aripiprazole-treated human neuroblastoma SH-SY5Y cells was performed. As a result, peroxiredoxin (Prx) 1 and 6, antioxidant enzymes, were identified in aripiprazole-treated SH-SY5Y cells. Subsequently, we examined the change in protein expression of antioxidant enzymes by aripiprazole. We observed a significant increase in Prx6 and superoxide dismutase (SOD) 1 protein expression in aripiprazole-treated SH-SY5Y cells compared to control SH-SY5Y cells. In contrast, there was no change in the expression of Prx1, SOD2, catalase and glutathione peroxidase. These results suggested that aripiprazole has an antioxidant effect on neurons via increased expression of Prx6 and SOD1.
