Studies were made on the permeability of dental enamel using albumen and dyestuffs. The results were as follows.
1) It was proved by precipitation reaction that albumen, applicated to the surface of normal enamel of dog's canine, penetrated the enamel and reached the pulp.
2) At least 24 hours duration of the application of the albumen was necessary for its penetration to the pulp.
3) The penetration was not accelerated by addition of glucose to the albumen.
4) The antibody for the alumen was not proved in the serum of each individual dog by precipitation reaction.
5) Each of the dyestuffs (5 % eosin, 3 % fuchsin, 1.5 phenol-fuchsin, 4 % methylene blue, 5 neutral red), applicated to the surface of normal enamel of dog's canine, penetrated also the enamel, and within the above mentioned dyestuff eosin, phenol fuchsin and methylene blue reached the pulp.
6) At least 20 hours duration of the application of the dyestuffs was necessary for its penetration to the pulp.
7) The direct relation was not found between the penetration of each dyestuff solution and its pH.
8) The penetration of the dyestuffs was easier in the region of the lingual fissure of enamel than other regions.