ESRスピントラップ法を用いたアスコルビン酸のスーパーオキシドラジカル消去能に関する実験的研究

抄録

The abilities of various concentrations (0 1000μM) of ascorbic acid to remove sup eroxide radicals were examined using the ESR spin trapping technique. Superoxide radicals were generated through the hypoxanthine-xanthine oxidase reaction system. Fifty pl of hypoxanthine, 35μl of DETAPAC, 50μl of ascorbic acid diluted to the desired concentrations, and 15μl of DMPO were placed in a test tube. Fifty μl of xanthine oxidase was added to the mixture and was mixed stirringly.ESR spectrometry was started just 1 minute after adding xanthine oxidase. The ESR spectrum obtained under the controlled condition using PBS showed the signals of DMPO -O_1/2 and DMPO-OH. The signals obtained at each concentration of ascorbic acid were normalized as the relati ve signal intensities for the standard signal intensity of manganese oxide marker. These relative signal intensities were plotted and evaluated.
The results showed that the relative signal intensities obtained at each concentration of ascorbic acid were decreased as the concentration of ascorbic acid was elevated from 0μM to 1000μM.