口腔カンジダ症患者由来Candida albicans株の分子生物型と生化学的性状

抄録

Fifty-three Candida albicans strains clinically isolated from 31 patients with oral candidosis were examined for the biochemical characteristics, molecular biological characteristics, and sensitivity to anti-fungal drugs. Biochemical characteristics were examined on production of chlamydospore at hyphal tip, carbohydrate assimilation pattern, extracellular proteinase activity and phospholipase. Molecular biological characteristics were assessed by means of the polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD) method. The sensitivity to anti-fungal drugs was analyzed MIC for amphotericin B, flucytosine, fluconazole, itraconazole, and miconazole.
All 53 strains produced chlamydospore on corn meal agar. Carbohydrate assimilation pattern showed many different patterns, and 49 strains of 53 clinically isolated yeasts were identified as Candida albicans. A number of variations were found among proteinase and phospho-lipase production on the plate method. Four of the 53 strains had high proteinase activity and five of the 53 strains showed high phospholipase activity.
RAPD typing methods could reveal differences and detected many different band patterns among the 53 strains of Candida albicans. At the MIC examination for anti-fungal drugs, 17 strains showed high resistance to fluconazole and they showed cross-resistance to itraconazole.
These results indicate that molecular biotyping offers more advantages in terms of simplicity and rapidity than previously established methods and may prove useful for surveying large numbers of pathogens in molecular epidemiological studies.