Abstract
Authors determined the titrable acid in the pyloric coeca extract, and also confirmed the existence of organic acid by using the method of paper partition chromatography with the following results.
1. In 100g of the pyloric coeca extract the total amount of acid was found to be 0.30g calculated as oxalic acid.
2. Four acids were ascertained to be present by paper the partition chromatographic method in the precipitates by lead acetate, Na2CO3 and CaCO3. But among these, oxalic acid and citric acid were detected together with two other unknown spots.
3. The existence of ether-soluble organic acid was not evidenced by this experiment.
4. Hydrazons obtained on adding 2, 4 dinitrophenyl hydrazine was examined by the paper partition chromatography. It was presumed that the following ketonic acids might be present, viz., a-keto glutaric acid, oxaloacetic acid, pyruvic acid and two unknown ketonic acids.
