Abstract
Amino acid pattern in acid hydrolyzate of the protein from the external mucous substance of 11 kinds of fishes (2 fresh water and 9 sea water fishes) was examined by paper chromatography. All the samples tested gave 16 spots of amino acids and 1 spot of unknown substance with ninhydrin (Fig. 1). As shown in Table 1, in which relative amounts of amino acids estimated roughly from the area and the color intensity of the spots are given, the mucous protein of these fishes seemed to be quite similar in amino acid composition except Dasyatis akajei, the protein of which differed considerably from that of the others, being poorer in leucine, alanine, phenylalanine and arginine, and richer in threonine. Raja hollandi was different from Dasyatis akajei in the amino acid composition, the former being rather similar to teleosts although they are both Elasmobranch fishes.
