Abstract
The purified cephalin fraction was obtained in the yield of 0.70% from the fresh pyloric appendages of Yellowtail, Seriola quinqueradiata.
The purified cephalin was precipitated fractionally after the way of FOLCH into five fractions, and the presence of phosphatidyl ethanolamine, phosphatidyl serine and a small amount of inositol phosphatide was confirmed from the analytical data on each fraction.
The characteristics of the fractionated cephalins are given in Table 1.
After the analysis, the rest of each fraction was mixed together following the proportion by which yielded from original cephalin, the fatty acid component was analyzed by alkaliisomerization which showed no presence of polyenoic fatty acids. According to simple calculation, the conjugated fatty acids of the cephalin consist of 29.22% saturated acids and 70.78% unsaturated acids.
The paper chromatography indicated the presence of myristic, palmitic, stearic, zoomaric, oleic, erucic, linolic and linolenic acids in the cephalin.
Sphingomyelin and cerebroside separated from the sphingolipid fraction by washing method of FOLCH et al., were obtained in the yield of 0.02% and 0.03% respectively, from the fresh samples.
