Abstract
To establish a simple polarographic method for determination of homarine occurring characteristically in marine invertebrates, extraction methods and supporting electrolytes were surveyed using the squid mantle muscle and liver.
Homarine in the tissues was easily extractable with 5-20 volumes of boiling water. The use of 1N H2SO4 as a supporting electrolyte was proposed, as it gave a well-defined first wave of homarine on polarography.
The amounts of homarine in the squid mantle muscle and liver were determined by the modified method, and a higher value was found in the liver than in the mantle muscle. The recovery of added homarine was satisfactory, ranging from 99 to 101%.
