Analysis of Fatty Acid Structures of Triglycerides by Preparative Gas-Liquid Chormatography
1976 Volume 42 Issue 2 Pages 227-231
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1976 Volume 42 Issue 2 Pages 227-231
Analytical method for triglyceride (TG) structures has been studied by using preparative gas-liquid chromatography (GLC) in order to apply the method to fish lipids, and the results obtained may be summarized as follows:
1. A column of 6mm i.d.×50cm, packed with 0.5% OV-1 (methyl silicone) on Shimalite W (80-100 mesh) was found suitable for the present purpose. The column temperature was programmed from 180° to 350°C with a rate of 6°C/min.
2. The efficiencies of 3 different sample collectors, each having 3 differenters, were compared. It was found that a simple glass tubing type of 3mm i.d. gave the highest recoveries.
3. Triglycerided in soybean oil was separated by preparative GLC. The TG fractions were hydrolyzed withe ethanol-pottasium hrdroxide to fatty acids, followed by cogversion of fatty acids to methyl esters with diazomethane, which were then analyzed by GLC. It was found that the TG of C-50 and C-52 in the oil were composed of palmitic, stearic anp oleic acids, and those of C-54, of stearic, oleic, linoleic and linolenic acids, respectively.
