Biochemical Studies on Carp Plasma Protein-I

Isolation and Nature of an Albumin

Abstract

The presence and nature of an albumin in carp plasma were investigated. It was estimated by cellulose acetate electrophoresis that the albumin was 34.6 per cent of the total plasma protein. The albumin obtained from carp plasma was a kind of lipoprotein with a molecular weight of approximately 15×10⁴, having a lipid content of 22.3 per cent of which 12.2 per cent consisted of free fatty acids. More than half of plasma lipid is conjugated with the albumin. The albumin evidently differs from the human type in its ability to bind BCG and BPB, molecular size, electrophoretic mobility, and nature of the lipid moiety.