Studies on the Yellow Spot Disease in Conchocelis-II
Method for Obtaining the Pathogen Complex
1977 Volume 43 Issue 3 Pages 255-258
Details
1977 Volume 43 Issue 3 Pages 255-258
For examining the factors influencing the development of the yellow spot disease in Porphyra conchocelis, it is essential that a pathogen which will attack the host positively be prepared with discretion, if in case the pathogen can not be purified. In this study, the methods for obtaining a pathogen complex was examined by centrifugation and filter-sterilization opaocedures. The results obtained are as follows:
1. It was ascertained that the pathogen complex exists mainly in the deritus deposits in the culture wawter of conchocelis, when the yellow spot disease is spreading strongly.
2. The pathogen complex existiong in the detritus is fraccionated in the supernatant at 6500xg and the precipitation at 1200xg by centrifugation.
3. It was proved by the methods of centrifugation and Zeitzfiltration of pathogenic water that the pathogen complex is an organism which is slightly smaller than normal bacteria and larger than virus.
4. In the laver culture industry, the yellow spot disease in porphyra conchocelis can possibly be prevented by eliminating the detritus from the cultre seawater at all times.
