Variation of Hematocrit Values of Rainbow Trout Blood Samples Incubated under Different Conditions

Abstract

Variation of hematocrit values was studied on rainbow trout blood samples incubated under different conditions of temperature, volume of blood sample and concentration of heparin sodium.
Blood samples were taken from the caudal artery with heparintreated syringes and transferred to two or three test tubes (7×70mm) in accordance with the purpose of the experiment.. Hematocrit values were determined at zero-time and after 30, 60, 90, 120 and 180 minutes for each sample. Ten samples were measured under each condition.
In Experiments 1 and 2, the effect of temperature was studied, change in hematocrit values became smaller at lower temperatures, and at 0.3°C the reading remained stable for 60 minutes after bleeding.
In experiment 3, the effect of the volume of blood in the test tube was studied. Each blood ample was divided into three parts, with volumes of 0.3, 0.6 and 0.9ml. Hematocrit values tended to become higher with the increase in blood volume in the test tube.
In Experiment 4, the effect of heparin concentration was studied. Within the range of 0.2-0.5mg heparin sodium per ml of blood, no effects of heparin concentration on hematocrit value were found.