A Viscous Antitumor Substance Obtained from a Marine Bacterium No. 9-12
1977 Volume 43 Issue 3 Pages 323-328
Details
1977 Volume 43 Issue 3 Pages 323-328
Amarine bacterium NO.9-12, isolated from sea water, produced an extracellular viscous substance in sea water medium.
The viscous substance was precipitated from the fermentation broth with acetone, extracted with M/100 EDTA and lyophilized after dialysis against water. The substance thus obtained exhibited antitumor activity for mouse sarcoma-180 (ascites type). Upon intraperitoneal injection of 1 mg/Kg/day ×5 and 5 mg/Kg/day ×5 of this substance, the growth of mouse ascites carcinoma was inhibited 84.4% and 87.2%, respectively.
Chemical analysis showed that the substance contained 25.8%C, 4.2%H, 6.1%N, and 27.3% ash.
The homogeneity of the crude extract was determined by colume chromatography on Sephadex G-75, Sephadex G-200 and Sepharose 6B, The data suggested that the polysaccharide was separated from the protein moiety and that the polysaccharide was heterogenous.
