Steroid Hormone Metabolites in Fish Urine-II
Identification of Pregnanediol, Pregnanertiol and Androstenediol Isolated from the Urine of Carp
1978 Volume 44 Issue 12 Pages 1399-1404
Details
1978 Volume 44 Issue 12 Pages 1399-1404
The present investigation deals with the identification of progesterone metabolites in the urine of carp, Cyprinus carpio. The urine sample collected by inserting a catheter into the urinary bladder was passed through an Amberlite XAD-2 column. The steroids adsorbed on the column were eluted with methanol and hydrolyzed with β-glucuronidase and by solvolysis (ethylacetate-1N H2S04), The liberated steroids were extracted with ethyl acetate and converted to methoxime-trimethylsilyl derivatives for gas liquid chromatographic (GLC) and mass spectrometric (MS) analyses.
The GLC separations were carried out on 1% OV-1 and 1% OV-l7 columns. The two peaks observed on the progesterone metabolite region and the one peak in the 17-ketosteroid region on the chromatograms were identified as those of pregnanediol, pregnanetriol, and androstenediol by methylene unit value determination. Their identities were also established by GLC-Mass spectrometry.
