Continuous Cell Line Derived from the Kidney of Yamame, Oncorhynchus masou

Abstract

A continuous cell line was established from the kidney of Yamame, Oncorhynchus masou. It took 8 weeks at 20°C and 9 weeks at 15°C in primary culture, 9-26 days from secondary to tenth culture, and 7-14 days since 10 passages for the surface of tissue culture bottle to be covered a confluent monolayer of cells. The cell line cultivated at 20°C from primary culture, designated YNK, has been subcultured 50 times over the period of 20 months. The cells of the line multiply in manner of fibroblastic monolayer with 65 chromosomes, and were characterized by the number of glanular intracytoplasmic organellas around nucleus. YNK cells have the permissible temperature range of 4-29°C for growth.