Preparation of Isolated Cells of Eel Liver

Abstract

Isolated cells of the eel liver were prepared from the liver perfused by the medium containing collagenase. Cells isolated by this method were undamaged when viewed with light microscopy; the viability of the cells was 85 to 90%, as judged by staining with 0.02% trypan blue. Adrenalin at 10^-4M promoted glycogenolysis in isolated cells, while insulin at 5×10^-5M significantly inhibited it. These cells actively incorporated ¹⁴C-pyruvate or ¹⁴C-lactate into glucose. Amino-oxyacetate (0.5mM) inhibited ¹⁴C-glucose synthesis not only from ¹⁴C-lactate but also from ¹⁴C-pyruvate. Respiratory inhibitors such as methylene blue (5×10^-3mM), KCN (1mM), and 2, 4-dinitrophenol (0.5mM) inhibited ¹⁴C-glucose synthesis from ¹⁴C-pyruvate by 58, 86, and 100% respectively.