1980 Volume 46 Issue 10 Pages 1203-1209
A continuous cell line was established from the embryonated eggs of chum salmon, Oncorhynchus keta, using conditioned medium, the filtered supernatant of rainbow trout gonadal cell line, RTG-2. It took 10 days in primary culture, 87 and 25 days in secondary and tertiary culture, respectively, and 7-10 days since fourth culture to form confluent cell sheets at 20°C. The cell line, designated SE, has been subcultured 150 times over the period of 3 years.
The cell line consists of fibroblastic spindle shaped cells and multiplies in an unrestrained fashion to form piled up colonies. Electron microscopically, desmosomes were often seen between two adjacent cells. And, numerous mitochondria were observed arround nuclei. The chromosome number of the cells was ranging from 53 to 79. This cell line has very wide permissible temperature range of 4-25° for growth.
SE cells had the lowest sensitivity to infectious pancreatic nectosis virus in four tested salmonid cell lines, RTG-2, YNK, and IK, which was originally established from the kidney of Iwana, Salvelinus leucomaeins, in our laboratory. And SE cells had as much sensitivity against infectious hematopoietic necrosis virus as those of RTG-2 and YNK cells.
