Effects of PCB on Lysosomes in the Hepatopancreas of Carp

Abstract

Investigation of the action of PCB on the carp hepatopancreas lysosomal membranes was carried out. The results obtained were as follows:
The fragility of hepatopancreas of carp 14 days after PCB administration was investigated by exposing the lysosome fraction by freezing and thawing or preincubation at 50°C. In all cases both hydrolase, acid phosphatase and β-glucuronidase were released in greater quantity than in the control specimens.
The release of acid phosphatase and β-glucuronidase were induced by the addition of PCB to the lysosome suspension prepared from normal carp hepatopancreas.
Sodium copper chlorophyllin, α-tocopherol, lecithin and cortisone inhibited the release of acid phosphatase and β-glucuronidase from the lysosome fraction of carp hepatopancreas in vitro.
It was suggested that PCB may induce instability of the lysosomal membrane depending on the lipophilic property.