Purification and Crystallization of a 320nm Absorbing Substance from the Red Alga, Chondrus yendoi

Abstract

Using a repeated active carbon column chromatography, a characteristic 320 nm UV-absorbing substance (X₁) from the red alga, Chondrus yendoi, was isolated as white crystals. It is very soluble in water, slightly soluble in methanol, and insoluble in all other organic solvents. its chemical formula was C₁₀H₁₆N₂O₅, mp 156-158°C (dec.), [Γ]a¬-7.9 (c=1.5, water), UV (water) _max 320 nm (log _ε=4.55), MS(FD) _m/_e 245 (MH⁺)