Abstract
Conditions for the heat inactivation of complements were investigated by heating normal sera and antisera, using masu salmon Oncorhynchus masou, rainbow trout Salmo gairdneri, coho salmon O. kisutch, goldfish Carassius auratus and tilapia Sarotherodon nilotica immunized with goldfish red blood cell (GFRBC) and rainbow trout red blood cell (RTRBC) as immunogens. The hemolytic complement activities against GFRBC or RTRBC were completely inactivated by the following heating conditions: 42°C-20min for masu salmon, 44°C-20min for rainbow trout, 45°C-20min for coho salmon and goldfish, and 47°C-20min for tilapia. Based on the heat inac-tivation of complements, immune hemolysis reaction was applied without trouble to the five fishes.
