Axenic Tissue Culture and Callus Formation of the Marine Brown Alga Laminaria angustata

Abstract

A method for axenic tissue culture and callus formation of the marine brown alga Laminaria angustata were established for the first time. Axenic medullary tissues were obtained from the stipes of the sporoplytes by the punching method using cork borer. Callus tissues have been induced from the medullary tissues on ASP 12-NTA medium solidified with 1.0% agar. These cultures were maintained in an incubator at 14°C and illuminated with cool white fluorescent lamps at ca. 2000 1x with 14 hr light and 10 hr dark cycle. This method for tissue culture will contribute to advances in breeding and cultivation of Laminaria plants.