The Composition of Bilirubin Conjugates in Piscine Biles

Abstract

The biles of carp Cyprinus carpio, eel Anguilla japonica, ayu Plecoglossus altivelis, yellowtail Seriola quinqueradiata, knifejaws Oplegnathus faciatus, and stingray Dasyatis akajei were analyzed for the composition of bilirubin conjugates. The ethyl anthranilate diazo method was used for determination of the total conjugated bilirubin, and TLC for analysis of the azopigments derived.
Bilirubin was for the most part present as the monoconjugate type in the biles of carp, knifejaws, and stingray, the proportion of this type to the total conjugated bilirubin being 72, 80, 80%m respectively. The corresponding proportion in the three other fishes and rat were 24-38%.
It was found that in the biles of carp, eel, ayu, yellowtail, knifejaws, and stingray, bilirubin glucuronide accounted for 34, 54, 49, 39, 13, and 14%, respectively, of the total conjugated bilirubin.