Histochemical Study on the Granulocytes in the Inflammation of Carp

Abstract

Histochemical examinations were made on the peroxidase, Mg-ATPase, and malate dehy-drogenase activities in the peripheral granulocytes, hematopoietic tissues and inflamed dorsal muscle tissues, using carp Cyprinus carpio inflamed with the injection of turpentine (1.0 and 0.1ml/kg body weight). The results obtained were very similar in the experiments of both the doses.
In the peripheral blood of the inflamed carp, the rate of the total granulocytes to the ery-throcytes and the rate of the granulocytes showing intense enzyme activities to those showing low enzyme activities begin to increase at 30 min and are in maxima at 2 days after the injection. In the hematopoietic tissues of the inflamed carp, the rate of the granulocytes showing intense peroxidase activity is in maximum at 2 days after the injection. These results may indicate that the granulocytes produced in the hematopoietic tissues are supplied gradually into the peripheral blood. This supply is slow in comparison to mammals. Therefore, the hematopoietic tissues of carps may not comtain large number of the matured granulocytes as seen in mammals.