Abstract
Intestinal absorption of cadmium (Cd) in vitro was measured using perfused intact segments of carp Cyprinus carpio (about 470g in body weight) intestine. Cd absorption rate at 25°C was about three times higher in posterior than in anterior segment. Absorption rate varied in pro-portion to Cd concentrations of 0.5 and 1.0μg/ml in the medium. But such proportion was not secn in other concentrations (1.5 and 1.8μg/ml). No difference in Cd absorption was noted between pH 7.3 and 9.3 of the media. However, absorption rate was approximately two times higher in the medium of pH 5.3 than in other media (pH 7.3 and 9.3). Absorption and transport increased remazkably with the rising of medium temperature from s to 25°C, and calculated Q10 values were in the range of 2.2 to 2.5. Inhibition in Cd absorption was seen and the inhibitory rate was about 30% using the following agents; 2, 4-dinitrophenol (DNP), ouabain, florizine and p-chloromercuritsenzoic acid (PCMB).
These results suggest that diffusion and active transport were involved in the process of Cd absorption in carp intestine.
