Abstract
×A mixture of phosphopeptides, obtained from the mature eggs of rainbow trout Salmo gairdneri by extraction with trichloroacetic acid and by precipitation with ethanol, was separated into 12 fractions by anion exchange chromatography using Dowex 1-2; 9 of them containing typical phosphopeptides were further separated into 2-3 fractions by gel chromatography using Sephadex G-50. Moleculaz weights of 15 phosphopeptides thus obtained ranged from 1, 100 to 2, 100 and from 4, 200 to 5, 500. Serine was the most prominent amino acid in all peptides, and phosphate and serine contents of each peptide were almost equimalar. On the basis of molecular weight, amino acid composition, and phosphate content, the consistution of phosphopeptides were char-acterized.
