Abstract
By using the myofibrils prepared from akazara scallop striated and smooth adductor muscles, dissociation of myosin regulatory light chains and the relation of the regulatory light chain con-tent to Mg-ATPase activity were investigated.
The regulatory light chain dissociation was found to occur more readily from the striated myofibrils than from the smooth ones. Moreover, it was confirmed that the complete removal of regulatory light chains was required for the complete loss of Ca-sensitivity of Mg-ATPase; and that the Mg-ATPase activity both in the presence and absence of Ca2+ changed in proportion to the regulatory light chain content. These results are very similar to those obtained with akazara myosin.
