Extraction of Phospholipid-Containing Feed Oil by Propylalcohol

Abstract

The traditional propylalcohol extraction procedure was re-examined from the view point of marine phospholipid recovery.
Thirty sardines were wholly minced and subjected to the 1-propanol extraction, 2-propanol extraction, and chloroform/methanol extraction as a control. A portion of minced material was freeze dried and it was also subjected to the 1-propanol extraction.
Lipids extracted by propylalcohols contained some amounts of non-lipid contaminants in the lipid extract, though they were easily removed by centrifuge (1500×g) from the extract.
1-Propanol extraction from the wet material was considered to be the most promising way to extract phospholipid among the commonly used nontoxic solvents, and it amounted to 70% of the chloroform/methanol extraction. Phosphatidylcholine was the most prominent component among the phospholipid classes in all the extracts.
Comparing the quality of the extracts, just a slight difference was observed in the molecular species composition of the main lipid classes, i. e. the phosphatidylcholine and the triglyceride. Among the molecular species of phosphatidylcholine of the extracts, (16:0, 22:6) was the most prominent combination, followed by (16:0, 20:5).
The molecular species of triglyceride in the extracts were so complicated that it was hard to point out the most prominent combination.