Production of Trigenomic Allotriploid Medaka Oryzias latipes sinensis-curvinotus-celebensis

Abstract

Hybrid females between Oryzias latipes sinensis and O. curvinotus lay non-reductional diploid eggs. We tried to produce trigenomic allotriploid medaka by fertilizing the diploid eggs with the sperm of three different species of Oryzias, O. celebensis, O. melastigma, and O. javanicus. Viable offspring were obtained when the eggs were inseminated by O. celebensis. Karyotype analysis confirmed that these fish were trigenomic allotriploids with three different genomes from O. latipes sinensis, O. curvinotus, and O. celebensis. Electrophoretic patterns of proteins (soluble muscle protein and eight isozymes) of these trigenomic allotriploids showed a normal expression of genes from these three genomes.