ニジマス肝臓のトリプトファンピロラーゼ

Augusto E. Serrano; 永山 文男

doi:10.2331/suisan.58.2367

Activity and Partial Purification of Liver Tryptophan Pyrrolase in Rainbow Trout

Augusto E. Serrano, Fumio Nagayama

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JOURNAL FREE ACCESS

1992 Volume 58 Issue 12 Pages 2367-2371

DOI https://doi.org/10.2331/suisan.58.2367

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Published: 1992 Received: June 03, 1992 Available on J-STAGE: February 29, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: February 29, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : Compounds that activate tryptophan pyrrolase in other animals like methylene blue, hematin, EDTA, and calciumion did not affect fish enzyme activity. Neutralized ascorbic acid showed an inhibitory effect.
Time-activity and enzyme concentration-activity curves were determined in three methods of assay.
Km was estimated via the Lineweaver-Burk plot to be 80m_M.
The enzyme was partially purified by a four-step process: solubilization, DEAE Sephadex A-50 chromatography (batch method), and by two consecutive DEAE Sepharose CL-6B chromatographs. The partially purified enzyme was highly unstable so that no characterization was at all possible.
Right : Compounds that activate tryptophan pyrrolase in other animals like methylene blue, hematin, EDTA, and calciumion did not affect fish enzyme activity. Neutralized ascorbic acid showed an inhibitory effect.
Time-activity and enzyme concentration-activity curves were determined in three methods of assay.
Km was estimated via the Lineweaver-Burk plot to be 80mM.
The enzyme was partially purified by a four-step process: solubilization, DEAE Sephadex A-50 chromatography (batch method), and by two consecutive DEAE Sepharose CL-6B chromatographs. The partially purified enzyme was highly unstable so that no characterization was at all possible.

Date of correction: February 29, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) N. C. Price and L. Stevens: Fundamentals of Enzymology, University of Stirling, Scotland, 1982, p. 135.
2) D. A. Bender: Amino Acid Metabolism, 2nd ed., Wiley Interscience Publ., New York, 1987, pp. 220-234.
3) R. T. Schimke, E. W. Sweeney, and C. M. J. Berlin: The stability of rat liver tryptophan pyrrolase. J. Biol, Chem. 240, 4609-4620 (1965).
4) K. W. Boehlke, R. L. Church, O. W. Tiemer, and B. E. Eleftheriou: Diural rhythm in plasma epinephrine and norepinephrine in the channel catfish. Gen. Comp. Endocrinol., 7, 18-21 (1966).
5) J. N. Brown and C. L. Dodgen: Fish liver tryptophan pyrrolase. Biochim. Biophys. Acta, 165, 463-469 (1968).
6) Y. S. Cho-chung and H. C. Pitot: Feedback control of rat liver tryptophan pyrrolase. J. Biol. Chem., 242, 1192-1198 (1967).
7) O. Greengard and P. Feigelson: The purification and properties of liver tryptophan pyrrolase. J. Biol. Chem., 237, 1903-1907 (1962).
8) W. E. Knox and A. H. Mehler: The conversion of tryptophan to hynurenine in liver. J. Biol. Chem., 187, 419-430 (1950).
9) A. A.-B. Badawy and M. Evans: Animal liver tryptophan pyrrolase. Biochem. J., 158, 79-88 (1976).
10) A. A.-B. Badawy and M. Evans: Guinea pig liver tryptophan pyrrolase. Biochem. J., 138, 445-451 (1974).
11) A. E. Serrano: Amino Acid Metabolism in Carp, Master's Thesis, Kyushu University, 1985.
12) A. E. Serrano and F. Nagayama: Inhibition studies on liver arylformamidases of rainbow trout and cattle. Comp. Biochem. Physiol., 98B, 281-285 (1991).
13) M. M. Piras and W. E. Knox: Tryptophan pyrrolase of liver. J. Biol. Chem., 242, 2952-2958 (1967).
14) Y. Ishimura: _L-Tryptophan 2, 3-dioxygenase (Pseudomonas), in “Methods in Enzymology”, (ed. by H. Tabor and C. W. Tabor), Vol. 17A, Academic Press, New York, 1980, pp. 429-433.
15) A. E. Serrano and F. Nagayama: Purification and characterization of liver arylformamidase in rainbow trout liver. Comp. Biochem Physiol., 98B, 381-387 (1991).
16) A. E. Serrano and F. Nagayama: Liver 3-hydroxyanthranilic acid oxygenase activity in rainbow trout. Comp. Biochem. Physiol., 98B, 275-280 (1991).
17) A. E. Serrano and F. Nagayama: Activity and stability of liver arylformamidase in fish. Nippon Suisan Gakkaishi, 57, 149-152 (1991).
18) W. E. Knox, A. Yip, and L. Reshef: _L-Tryptophan 2, 3-oxygenase (rat liver), in “Methods in Enzymology” (ed. by H. Tabor and C. W. Tabor), Vol. 17A, Academic Press, New York, 1980, pp. 415-421.

Right : 1) N. C. Price and L. Stevens: Fundamentals of Enzymology, University of Stirling, Scotland, 1982, p. 135.
2) D. A. Bender: Amino Acid Metabolism, 2nd ed., Wiley Interscience Publ., New York, 1987, pp. 220-234.
3) R. T. Schimke, E. W. Sweeney, and C. M. J. Berlin: The stability of rat liver tryptophan pyrrolase. J. Biol, Chem. 240, 4609-4620 (1965).
4) K. W. Boehlke, R. L. Church, O. W. Tiemer, and B. E. Eleftheriou: Diural rhythm in plasma epinephrine and norepinephrine in the channel catfish. Gen. Comp. Endocrinol., 7, 18-21 (1966).
5) J. N. Brown and C. L. Dodgen: Fish liver tryptophan pyrrolase. Biochim. Biophys. Acta, 165, 463-469 (1968).
6) Y. S. Cho-chung and H. C. Pitot: Feedback control of rat liver tryptophan pyrrolase. J. Biol. Chem., 242, 1192-1198 (1967).
7) O. Greengard and P. Feigelson: The purification and properties of liver tryptophan pyrrolase. J. Biol. Chem., 237, 1903-1907 (1962).
8) W. E. Knox and A. H. Mehler: The conversion of tryptophan to hynurenine in liver. J. Biol. Chem., 187, 419-430 (1950).
9) A. A.-B. Badawy and M. Evans: Animal liver tryptophan pyrrolase. Biochem. J., 158, 79-88 (1976).
10) A. A.-B. Badawy and M. Evans: Guinea pig liver tryptophan pyrrolase. Biochem. J., 138, 445-451 (1974).
11) A. E. Serrano: Amino Acid Metabolism in Carp, Master's Thesis, Kyushu University, 1985.
12) A. E. Serrano and F. Nagayama: Inhibition studies on liver arylformamidases of rainbow trout and cattle. Comp. Biochem. Physiol., 98B, 281-285 (1991).
13) M. M. Piras and W. E. Knox: Tryptophan pyrrolase of liver. J. Biol. Chem., 242, 2952-2958 (1967).
14) Y. Ishimura: L-Tryptophan 2, 3-dioxygenase (Pseudomonas), in “Methods in Enzymology”, (ed. by H. Tabor and C. W. Tabor), Vol. 17A, Academic Press, New York, 1980, pp. 429-433.
15) A. E. Serrano and F. Nagayama: Purification and characterization of liver arylformamidase in rainbow trout liver. Comp. Biochem Physiol., 98B, 381-387 (1991).
16) A. E. Serrano and F. Nagayama: Liver 3-hydroxyanthranilic acid oxygenase activity in rainbow trout. Comp. Biochem. Physiol., 98B, 275-280 (1991).
17) A. E. Serrano and F. Nagayama: Activity and stability of liver arylformamidase in fish. Nippon Suisan Gakkaishi, 57, 149-152 (1991).
18) W. E. Knox, A. Yip, and L. Reshef: L-Tryptophan 2, 3-oxygenase (rat liver), in “Methods in Enzymology” (ed. by H. Tabor and C. W. Tabor), Vol. 17A, Academic Press, New York, 1980, pp. 415-421.

Date of correction: February 29, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Compounds that activate tryptophan pyrrolase in other animals like methylene blue, hematin, EDTA, and calciumion did not affect fish enzyme activity. Neutralized ascorbic acid showed an inhibitory effect.
Time-activity and enzyme concentration-activity curves were determined in three methods of assay.
Km was estimated via the Lineweaver-Burk plot to be 80mM.
The enzyme was partially purified by a four-step process: solubilization, DEAE Sephadex A-50 chromatography (batch method), and by two consecutive DEAE Sepharose CL-6B chromatographs. The partially purified enzyme was highly unstable so that no characterization was at all possible.

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