Abstract
The mutagenicity of 19 chlorohydrins, including 3-chloro-1, 2-propanediol, and 4 protein hydrolysates which contained 3-chloro-1, 2-propanediol, were demonstrated with two Salmonella mutation assays, ultramicro forward-mutation assay using TM677 in the presence and absence of a metabolic activation system (S9mix) and preincubation assay using TA100 and TA98 in the presence and absence of S9mix.
As a result, 11 chlorohydrins and 3 protein hydrolysates showed mutagenicity. Three dichloroalkanols were more mutagenic than 3-chloro-1, 2-propanediol. Eight monochloroalkanols showed mutagenicity. Optical isomers of 3-chloro-1, 2-propanediol and 2-chloro-1-propanol showed different mutagenic activities. It was suggested that the hydrolysates contained not only 3-chloro1, 2-propanediol but also other mutagens.
