Abstract
DNA fingerprinting and isozyme analyses of 32 Ginbuna (all female triploids) collected from the Monobe River, Kochi prefecture were carried out for identification of clones.
Genomic DNA extracted from blood were digested with Hae III restriction endonuclease, and were hybridized with 32P-labeled YNZ 22 probe for DNA fingerprinting. Isozymes of muscle and liver frozen at -20°C were assayed by the horizontal starch gel electrophoresis to detect creatine kinase (CK), esterase (EST), glucosephosphate isomerase (GPI), phosphoglucomutase (PGM), general protein (PROT).
Specimens were classified into 7 clonal groups by DNA fingerprinting. The mean of band sharing index (BSI) were 0.314±0.330 between individuals, 1.000 between individuals within a clone, and 0.149±0.077 between different clonal lines. The isozyme patterns were also useful for identification of the clones detected by DNA fingerprinting. There were no significant differences in morphological characters between clones of Ginbuna except two morphological traits.
