Determination of Benzene and Phenol in Body Fluids by Headspace Solid-Phase Microextraction (SPME. and Capillary Gas Chromatography

抄録

Benzene and its metabolite phenol are extractable from human whole blood and urine using headspace solid-phase microextraction (SPME. with a Carboxen/polydimethylsiloxane fiber. Both compounds were assayed using ethylbenzene and 2,4-dimethylphenol as internal standards (IS. and capillary gas chromatography (GC. with flame ionization detection. The headspace SPME-GC gave sharp peaks for benzene, phenol, and IS-1, 2; with the whole blood and urine samples showing low background noise. The extraction efficiencies of benzene and phenol were 51.8–99.4% and 10.6–14.3%, respectively, for both whole blood and urine. Regression equations also showed excellent linearity in the range of 5–400ng/0.5ml for benzene and 10–500ng/0.5ml for phenol extracted from whole blood and urine. The detection limits (signal-to-noise ratio=3. for the benzene and phenol were 5–10ng/0.5ml for whole blood and 2–5ng/0.5ml for urine. The coefficients of within-day variation in terms of extraction efficiency for both compounds in whole blood and urine samples were not greater than 13.8%. These data indicate that benzene and phenol can be successfully separated and determined from human samples using the established headspace SPME method.