抄録
An immunoelectron microscopic study using a preembedding double Immunostaining technique was applied to examine synaptic relations between catecholaminergic neurons and GABAergic neurons in the area postrema of the rat. As a supplement to our previous report1), both anti-dopamine- β -hydroxylase and anti-phenylethanolamine-N-methyltransferase antisera were used to identify the different catecholaminergic neurons. Similar to our previous study1), there were reciprocal synaptic relationships between GABAergic neurons and noradrenergic or adrenergic neurons. Although tyrosine-hydroxylase immunoreactive perikarya had often been observed postsynaptic to GABAergic axon terminals, neither dopamine-β-hydroxylase nor phenylethanolamine-N-methyltransferase immunoreactive perikarya were found to receive synapses from GABAergic axon terminals. Instead, dopa-mine-β-hydroxylase immunoreactive axon terminals were often found to make synapses on GABAergic perikarya, whereas no axo-somatic synapses were found between phenylethanolamine-N-methyltransferase immunoreactive neurons and GABAergic neurons.
