抄録
Expansion and maintenance of hematopoietic progenitor cells (BFU-E, CFU-GM) ex vivo were examined using bone marrow mononuclear cells obtained from children with malignant solid tumors and acute leukemias during the recovery phase of bone marrow after chemotherapy. Bone marrow mononuclear cells were cultured in serum-free liquid culture system containing stem cell factor (SCF), IL-3, and IL-6. The number of hematopoietic progenitor cells before the starting of liquid culture (day 0) was compared with that of colonies derived from progenitor cells after liquid cultures which contained various combinations of cytokine (day 7) . Viability of mononuclear cells on day 7 showed a 55% decrease when cultured without cytokine and the maximum of a 134% increase when cultured with cytokine. The number of CFU-GM increased 4.2-10.9 fold (range), 7.8±4.9 (mean±SD) and BFU-E increased 4.2-10.9 fold (range), 7.8±4.9 (mean±SD) in the presence of IL-3 +IL-6+SCF. The results indicate that it is possible to expand and maintain ex vivo the hematopoietic progenitor cells of bone marrow taken during the recovery phase after conventional chemotherapy from pediatric patients with malignant solid tumors and acute leukemias.
