We examined the effects of several detergents on the stability of halophilic Nesterenkonia halobia α-amylase（NhAmy）. NhAmy was purified to homogeneity from the culture supernatant by simple one-step dextrin affinity column chromatography. The purified enzyme was found to be highly resistant to several detergents, including sodium dodecyl sulfate, which is one of the strongest protein denaturants.
