1986 Volume 21 Issue 6 Pages 545-550
A micro-analytical method for trace amount of benzo (a) pyrene (Ba P) in airborne particulates by low temperature spectrofluorometry was developed. This method consisted of ultrasonic extraction, liquid-liquid partitions by using 5% sodium hydroxide aqueous solution and 20% sulfuric acid, successively, and direct determination of Ba P by using specrofluorometry at 77K with narrow baseline method. In the spectrofluorometric determination, excitation wavelengths were set at 368 nm and 309 nm, fluorescence spectra were measured from 398 nm to 408 nm, and fluorescence peak height was obtained by the narrow baseline method. Ba P concentration was calculated by simultaneous equations to eliminate the interfering efect bybenzo (k) fluoranthene (and other contaminants) in the sample. Ba P concentrations in airborne particulates analyzed by this method agreed with those by HPLC spectrofluorometry.
