Studies of the therapeutic effects of hyperthermia and various anticancer drugs on the cultured human Gallbladder cancer cell line

Abstract

Examining the effect of ADM, MMC and 5-FU on the Nozawa (Cultured Human Gallblader Cell lines) at the hyperthermic conditions, ADM treated group showed most prominent enhanced inhibitory effect to the Nozawa by the hyperthermic treatment.
Examining the effects of combined treatment of hyperthermia and anticancer drugs on the Nozawa cells by changing the incubation period, the ADM treated group showed prominent enhanced inhibitory effect by elonging the incubation period.
Examining the correlations between the CEA production and the cell viability by the combined treatment of hyperthermia and anticancer drugs, the CEA production showed no positive correlation with the cell viavility.
Hyperthermia decreased the cell viavility, however, it did not effect the CEA production.
The changes of therapeutic effects of anticancer drugs at the hyperthermic conditions observed in these results probably due to the differrences of pharmacological mechanism of action as well as the permeability of cell membrance.