日本におけるマイマイサンゴムシ属（新称）Brachylaima 吸虫未同定種の報告ならびに既知種の新産地・新宿主

抄録

From 2018 to 2021, land snail surveys were carried out from Okinawa to Hokkaido, Japan to find metacercariae of the trematode genus Brachylaima. When metacercariae were obtained from land snails, their morphologies were recorded and partial sequences of mitochondrial DNA (cox1) and nuclear 28S rDNA were determined for DNA barcoding. As a p-distance value of less than 1.8% was considered to be intraspecific variation for cox1 (630 bp) based on the sequences of known species of this genus, the metacercariae obtained in this study were discriminated into five putative species. Two of the five putative species were molecularly identified to two known species, B. lignieuhadrae and B. phaedusae, respectively, whereas the remaining three were treated as unidentified species. It is unknown whether the unidentified metacercariae were larvae of known species without genetic information or new species. Morphological and molecular comparisons of adults are necessary for the taxonomic clarification of these unidentified metacercariae.