It was found that azalomycin B (I), which was produced by a species of Streptomyces and showed inhibition against gram-positive bacteria, was a kind of macrolide antibiotics having a composition of C_<52>H_<92>O_<19> as a tentative formula. The sugar component of I was designated as 2-deoxy-l-fucose (VIII). Alkaline degradation of I or its derivative suggested the existence of the carbon chain like XV in the aglycon part of the antibiotic.