An acidic lipophilic antibiotic was isolated from the fermentea broth of the Streptomyces sp. No. 8403-MC_1. The antibiotic was identified with PA-132. Based on the chemical and spectroscopic evidences, it has an unique pentalene skeleton with a seven membered glycidic lactone as shown in Fig. 3 and was named pentalenolactone. The X-ray analisis of pentalenolactone 2-bromoethylamine salt is now under investigation.