Seven triterpenoid glycosides and two phenylpropanoid glycosides were isolated from the fruits of Ilex rotunda. These compounds were determined based on UV, MS, IR ^1H-NMR, ^<13>C-NMR and 2D-NMR spectroscopic data as 3-O-β-glucuronopyranosyl oleanolic acid [1], 3-O-β-glucuronopyranosyl-olean-12-ene-28-oic acid 28-O-β-glucopyranoside [2], 3-O-β-glucuronopyranosyl-23-hydroxyl methyl olean-12-ene-28-oic acid [3], 23-hydroxyl methyl olean-12-ene-28-oic acid [4], 3-O-β-glucuropyranosyl-19-O-β-glucopyranosyl-23-hydroxyl methyl olean-12-ene-28-oic acid [5], 3-O-β-glucuronopyranosyl-19-hydroxy-23-hydroxyl methyl olean- 12-ene-28-oic acid 28-O-β-glucopyranoside [6], 19-hydroxy-olean 12-ene-28-oic acid [7], syringin [8] and chlorogenic acid [9]. And we examined their superoxide scavenging activity and tyrosinase inhibitory activity (Step 1 as L-Tyrosine substrate; Step 2 as L-DOPA substrate), and their compounds investigated for cosmetic use. As a result, for superoxide scavenging activity, the 1-butanol extract and aqueous phase part were good activity of 69.30 and 72.22%, respectively. For tyrosinase inhibitory activity, the 1-butanol extract, good inhibition of 45.05% of Step 1 (L-Tyrosine substrate) was noted. Melanin inhibition and cytotoxicity were examined by using cultured B16 melanoma cells. More than 60% melanin inhibition was observed in the 1-Hexan extract, and methanol eluate of an Amberlite XAD-2. In addition, remarkable melanin inhibitions were found in [1] and [5] which are those isolation compounds. Among these isolated compounds, [5] is a new triterpenoid.
