In Japanese Industrial Standards (JIS), the method of using iron base binary calibration curve is adopted for X-ray fluorescence analysis of steels, and is defined to be applicable to the analysis of nickel base alloys. In this report, some problems were studied for applying the JIS method to the analysis of practical nickel alloy samples. Also, the correction factors of interelement effects suitable for those practical samples were determined. Moreover, the method of using nickel base binary calibration curve was compared with that of using iron base binary calibration curve, the JIS method, for the analysis of nickel base alloys. As the result, the accuracy of both methods had no difference and it was comfirmed that the method of using iron base binary calibration curve was applicable to nickel base alloys, even if the content of iron was less than 10%.