1986 Volume 2 Issue 2 Pages 199-204
The in vitro antiproliferative effect of recombinant murine interferon-β (Rec- MuIFN-β) combined with hyperthermia against mouse malignant glioma was evaluated. An increase in temperature enhanced the antiproliferative of Rec-MuIFN-β in the range of 40°C-43°C. In order to probe the mechanism of the enhanced antiproliferative effect, prostaglandin E2 and 2 - 5A synthetase activities in the culture medium were measured. Rec-MuIFN-β treatment resulted in greater prostaglandin E2 and 2-5A synthetase induction at 43°C than at 37°C. Flowcytometry studies of the DNA histogram showed that combined therapy prolonged the accumulation in the G2M phase. Thus, these cellular effects resulting from Rec-MuIFN-β were enhanced by elevated temperature. These results indicate the possibilities of a significant antitumor effect produced by the combination therapy of interferon and hyperthermia against human malignant gliomas.
