Abstract
Membrane microdomains (lipid rafts) are cholesterol- and glycosphingolipid-enriched lipid domains, which are involved in various cellular responses connecting the intracellular and extracellular environments. We investigated the neutrophilic function focusing on lactosylceramide-enriched membrane microdomains on the neutrophilic cell plasma membrane. Although membrane microdomains in neutrophils are known to be involved in neutrophilic functions, such as phagocytosis, the molecular mechanisms of action remain to be elucidated. In this review, strategies for comprehensive analysis of the microdomain-associated proteins for investigating the molecular mechanisms of action of these domains are described.
