Trends in Glycoscience and Glycotechnology
Online ISSN : 1883-2113
Print ISSN : 0915-7352
ISSN-L : 0915-7352
Article for JSCR 40th Anniversary Issue
NMR Study of Glycan Interactions Using Synthetic Oligosaccharides
Shinya Hanashima
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2019 Volume 31 Issue 181 Pages SE67-SE68

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Abstract

Recent advances in synthesizing methodologies and purification techniques significantly increase the accessibility of the major glycan structures, which are heterogeneously present on the glycoproteins and glycolipids. In contrast, it is often difficult to obtain sufficient amounts of minor structures consisting of a number of sugar units with reasonable purity sufficient for biological studies. In terms of the accessibility of the desired glycan sequences, it is useful in certain instances to deal with not the full sequence but with the required oligosaccharides, called glycan epitopes. Glycan epitopes have been used for the structural and functional analysis of the proteins that recognize particular glycans. For instance, lectins usually recognize the glycan epitope of a few sugar units, not in a “key-and-keyhole” manner but in a “pattern recognition receptor” mode. Such oligosaccharide epitopes can be prepared through regular chemical synthesis. This report describes recent progress on the NMR analysis of glycan–glycan and protein–glycan interactions using chemically synthesized glycan epitopes.

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© 2019 FCCA (Forum: Carbohydrates Coming of Age)
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