Endo-β-xylosidase Activity of Cellulase from Aspergillus niger for the Carbohydrate Chain Analysis of Proteoglycan

Abstract

Cellulase from Aspergillus niger (EC. 3.2.1.4) has endo-β-xylosidase activity. This enzyme acts on the xylose-serine linkage in the linkage structure between the core peptide and the glycosaminoglycan of the peptidoglycan which was obtained by protease treatment of proteoglycan, resulting in the release of glycosaminoglycan with xylose at the reducing end. Owing to this activity, it is possible to analyze glycosaminoglycan with long chains. This mini review introduces the endo-β-xylosidase activity of cellulase and its utilization.